巴氏芽孢杆菌诱导钙锰共沉淀的动力学研究开题报告

（1）YE-urea培养基（100 mL）：

锥形瓶①：酵母粉2 g，Tris0.7875 g，琼脂粉1 g，去离子水50 mL。

4. 参考文献

1.Fang, L., Q. Niu, L. Cheng, J. Jiang, Y.-Y. Yu, J. Chu, V. Achal, and T. You, Ca-mediated alleviation of Cd2 induced toxicity and improved Cd2 biomineralization by Sporosarcina pasteurii. Science of The Total Environment, 2021. 787: 147627.
2.Hu, L., H. Wang, P. Xu, and Y. Zhang, Biomineralization of hypersaline produced water using microbially induced calcite precipitation. Water Research, 2021. 190: 116753.
3.Wang, M., S. Wu, J. Guo, X. Zhang, Y. Yang, F. Chen, and R. Zhu, Immobilization of cadmium by hydroxyapatite converted from microbial precipitated calcite. Journal of Hazardous Material, 2019. 366: 684-693.
4.王新花, 赵晨曦, 潘响亮, 基于微生物诱导碳酸钙沉淀(MICP)的铅污染生物修复. 地球与环境, 2015. 43(1): 80-85.
5. Zhao, J., L. Csetenyi, and G.M. Gadd, Fungal-induced CaCO3 and SrCO3 precipitation: a potential strategy for bioprotection of concrete. Science of The Total Environment, 2021: 151501.
6. Kang, B., F. Zha, W. Deng, R. Wang, X. Sun, and Z. Lu, Biocementation of pyrite tailings using microbially induced calcite carbonate precipitation. Molecules, 2022. 27(11).
7.Hatayama, K., Manganese carbonate precipitation induced by calcite-forming bacteria. Geomicrobiology Journal, 2020. 37(7): 603-609.
8.Cuaxinque-Flores, G., J.L. Aguirre-Noyola, G. Hernndez-Flores, E. Martnez-Romero, Y. Romero-Ramrez, and O. Talavera-Mendoza, Bioimmobilization of toxic metals by precipitation of carbonates using Sporosarcina luteola: An in vitro study and application to sulfide-bearing tailings. Science of The Total Environment, 2020. 724: 138124.
9.Li, Q., D. Liu, Z. Jia, L. Csetenyi, and G.M. Gadd, Fungal biomineralization of manganese as a novel source of electrochemical materials. Current Biology, 2016. 26(7): 950-955.
10.Mahadevan, G., Q. Ruifan, Y.H. Hian Jane, and S. Valiyaveettil, Effect of polymer nano- and microparticles on calcium carbonate crystallization. ACS Omega, 2021. 6(31): 20522-20529.
11.王茂林, 吴世军, 杨永强, 陈繁荣, 微生物诱导碳酸盐沉淀及其在固定重金属领域的应用进展. 环境科学研究, 2018. 31(2): 206-214.
12.Sm, A., A. Oc, A. Cd, A. So, B. Vz, and B. Mva, An indigenous bacterium with enhanced performance of microbially-induced Ca-carbonate biomineralization under extreme alkaline conditions for concrete and soil-improvement industries. Acta Biomaterialia, 2021. 120: 304-317.

5. 计划与进度安排

1、2024.12.19~2024.1.8 查阅文献，确定课题内容框架；学习巴氏芽孢杆菌培养的基本操作。
2、2024.1.9-2024.3.5 完成文献翻译。调研大量文献，完成开题报告。设计实验方案并完成预实验。
3、2024.3.6-2024.5.7 探究不同钙离子浓度对菌诱导锰沉淀的动力学影响：表征pH、电导率、脲酶活性、锰去除率、沉淀干重等；利用显微成像对锰沉淀进行形态学分析；总结钙离子浓度对MICP固定锰的影响，确定最优浓度；对所得数据进行处理并画图；完成论文主体内容的撰写。
4、2024.5.8-2024.5.21 补充相关实验内容，对实验结果进行总结分析；完善毕业论文，准备毕业答辩。